产品货号：
Z20089
中文名称：
MCM4抗体
英文名称：
Anti-MCM4 Antibody
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-MCM4 Antibody |
| 指标别名 | CDC21;CDC21 homolog;CDC54;hCdc21;MCM4;P1 CDC21 |
| 克隆性 | Polyclonal |
| 检验物种 | human,mouse,rat |
| 应用范围 | WB,IHC,ICC/IF,FCM,ELISA |
| 基因名称 | MCM4 |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | E.coli-derived human MCM4 recombinant protein (Position:M361-A677). |
| 实际分子量 | 97KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | MCM4(MINICHROMOSOME MAINTENANCE,S.CEREVISIAE,HOMOLOG OF,4),also called CDC21,S.POMBE,HOMOLOG OF,is a protein that in humans is encoded by the MCM4 gene.MCM4 is one of the highly conserved mini-chromosome maintenance proteins(MCM) that are essential for the initiation of eukaryotic genome replication.The MCM4 gene is mapped to 8q11.21.The 864-amino acid MCM4 protein has an observed molecular mass of 97kD by SDS-PAGE,which is similar to its calculated molecular mass of 96.6kD.Western blot analysis with and without phosphatase treatment suggested that MCM4 is highly phosphorylated in mitotic cells.In the absence of DDK,CDK phosphorylation at the distal part of the Mcm4 NSD becomes crucial.MCM4 encodes a subunit of the MCM2-7 complex(also known as MCM2-MCM7),the replication licensing factor and presumptive replicative helicase. |
| Uniprot ID | P33991 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot. |
| 基因名全称 | minichromosome maintenance complex component 4 |
| 蛋白名全称 | DNA replication licensing factor MCM4 |
| 推荐稀释比 | Western blot (WB) 1:1000～5000 Immunohistochemistry (IHC) 1:50～400 Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50～400 Flow cytometry (FCM) 1～3μg/1×106 cells Enzyme linked immunosorbent assay (ELISA) 1:100～1000 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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